Arachidonic Acid–Induced Dilation in Human Coronary Arterioles: Convergence of Signaling Mechanisms on Endothelial TRPV4-Mediated Ca Entry
نویسندگان
چکیده
TRPV4 antagonist RN-1734 and by inhibition of endothelial Ca-activated K channels. In native and TRPV4-overexpressing human coronary artery ECs (HCAECs), AA increased intracellular Ca concentration ([Ca]i), which was mediated by TRPV4-dependent Ca entry. The AA-induced [Ca]i increase was inhibited by cytochrome P450 (CYP) inhibitors. Surprisingly, the CYP metabolites of AA, epoxyeicosatrienoic acids (EETs), were much less potent activators of TRPV4, and CYP inhibitors did not affect EET
منابع مشابه
Arachidonic Acid–Induced Dilation in Human Coronary Arterioles: Convergence of Signaling Mechanisms on Endothelial TRPV4‐Mediated Ca2+ Entry
BACKGROUND Arachidonic acid (AA) and/or its enzymatic metabolites are important lipid mediators contributing to endothelium-derived hyperpolarizing factor (EDHF)-mediated dilation in multiple vascular beds, including human coronary arterioles (HCAs). However, the mechanisms of action of these lipid mediators in endothelial cells (ECs) remain incompletely defined. In this study, we investigated ...
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Bubolz AH, Mendoza SA, Zheng X, Zinkevich NS, Li R, Gutterman DD, Zhang DX. Activation of endothelial TRPV4 channels mediates flow-induced dilation in human coronary arterioles: role of Ca entry and mitochondrial ROS signaling. Am J Physiol Heart Circ Physiol 302: H634–H642, 2012. First published December 2, 2011; doi:10.1152/ajpheart.00717.2011.—In human coronary arterioles (HCAs) from patient...
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